| omentin

Endagglutinin 1(ITLN1), also known as Omentin or intestinal lactoferrin receptor, is a protein encoded by the ITLN1 gene. It is both a receptor for the bacterium arabinogalactose and a receptor for lactoferrin. It enhances insulin-stimulated glucose uptake in fat cells and is a link between obesity and type 2 diabetes. It also has the function of blood vessel dilation, which plays a protective role during coronary atherosclerosis and hypertension.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.31	1.65	1.06	0.69	0.44	0.32	0.23	0.1
Corrected OD	2.21	1.55	0.96	0.59	0.34	0.22	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human omentin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	94-107	102
Tissue Homogenates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human omentin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-97	88-96	90-110
	Average (%)	93	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108